[email protected] (受疫情影响,东南亚目前只开放曼谷诊所)
全周 (9AM - 5PM)

我们和你在一起

Extra info thumb
  • 总部: 曼谷帕图万拉玛1路44号斯里朱尔苏普大楼22楼
  • pansmed
POLYAMINE OXIDASE; PAOX

POLYAMINE OXIDASE; PAOX

Alternative titles; symbolsPAOHGNC Approved Gene Symbol: PAOXCytogenetic location: 10q26.3 Genomic coordinates (GRCh38): 10:133,379,261-133,391,693 (from NCB...

Alternative titles; symbols

  • PAO

HGNC Approved Gene Symbol: PAOX

Cytogenetic location: 10q26.3 Genomic coordinates (GRCh38): 10:133,379,261-133,391,693 (from NCBI)

▼ Description
The polyamines spermidine and spermine are assembled by sequential transfer of aminopropyl units onto a core diamine unit, putrescine. In the polyamine back-conversion pathway, the same aminopropyl units are removed from spermine and spermidine by sequential acetylation followed by oxidation. PAOX is an FAD-dependent polyamine oxidase (EC 1.5.3.11) that functions in the polyamine back-conversion pathway (summary by Vujcic et al., 2003).

▼ Cloning and Expression
By searching databases for sequences similar to human spermine oxidase (SMOX; 615854), Vujcic et al. (2003) identified PAOX, which they called PAO. The deduced 511-amino acid protein has a calculated molecular mass of 55.5 kD and shares 39% identity with SMOX. Vujcic et al. (2003) also identified mouse Pao, which encodes a deduced 504-amino acid protein that shares 81% identity with human PAO. EST database analysis detected PAO expression in brain, eye, kidney, ovary, and prostate, as well as in several neoplasias.

Wu et al. (2003) determined that the mouse Pao protein has 2 conserved FAD-binding regions and a C-terminal peroxisomal targeting signal sequence. PCR analysis detected variable Pao expression in all adult mouse tissues examined, with highest expression in liver and stomach. Expression of Pao increased with development in embryonic mice.

▼ Gene Function
Using transfected HEK293 cells, Vujcic et al. (2003) found that human PAO showed FAD-dependent oxidation of N(1)-acetylspermine to form spermidine and that it released 3-acetamidopropanal and hydrogen peroxide. PAO showed similar kinetics in the oxidation of N(1)-acetylspermidine to form putrescine. With monoethyl- and diethylspermine analogs, PAO cleaved the substrate internally and adjacent to a secondary amine. PAO showed much weaker activity in the oxidation of N(1),N(12)-diacetylspermine and very low activity against spermine. Spermidine was not a PAO substrate. Mouse Pao showed similar kinetics and substrate preferences.

Independently, Wu et al. (2003) found that recombinant mouse Pao oxidized N(1)-acetylspermine to spermidine and 3-acetamidopropanal. It also oxidized N(1)-acetylspermidine to putrescine and 3-acetamidopropanal.

▼ Mapping
By genomic sequence analysis, Vujcic et al. (2003) mapped the PAOX gene to chromosome 10.

Hartz (2014) mapped the PAOX gene to chromosome 10q26.3 based on an alignment of the PAOX sequence (GenBank AL834535) with the genomic sequence (GRCh37).

Wu et al. (2003) stated that the mouse Paox gene maps to chromosome 7F4.

Tags: 10q26.3

相关文章

POLYAMINE OXIDASE; PAOX
ENOYL-CoA HYDRATASE, SHORT-CHAIN, 1, MITOCHONDRIAL; ECHS1

Alternative titles; symbolsSH...

POLYAMINE OXIDASE; PAOX
激酶非催化 C-LOBE 结构域蛋白 1

KNDC1 是 Ras 小 GTP 酶的鸟嘌呤核苷酸交换因...

POLYAMINE OXIDASE; PAOX
蛋白磷酸酶 2,调节亚基 B,德尔塔

蛋白磷酸酶 2A (PP2A) 是一种多聚体丝氨酸/苏氨酸...

POLYAMINE OXIDASE; PAOX
细胞色素 P450,亚家族 IIE

CYP2E1 基因编码一种酶 ( EC 1.14.13.-...

POLYAMINE OXIDASE; PAOX
肌苷多磷酸5-磷酸酶,40-KD

磷脂酰肌醇是许多不同信使分子的前体。激动剂刺激细胞导致磷脂...

遇到问题了吗?