Alternative titles; symbolsZK1HGNC Approved Gene Symbol: ZNF443Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38): 19:12,429,705-12,441,020 (from NCB...
Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF443
Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38): 19:12,429,705-12,441,020 (from NCBI)
Zinc finger proteins (ZNFs) bind DNA and, through this binding, regulate gene transcription. Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. For a general description of these proteins, see ZNF91 (603971).
▼ Cloning and Expression
Katoh et al. (1998) cloned a ZK1 cDNA from a human leukemia cell line by RT-PCR using degenerate primers corresponding to conserved regions of zinc finger proteins. The full-length cDNA clone encodes a deduced 671-amino acid protein with a Kruppel-associated box (KRAB) type A at its N terminus, 15 consecutively repeated ZNF motifs at its C terminus, and 2 incomplete ZNF motifs. Northern blot analysis detected ZK1 mRNA in almost all tissues tested; highest levels were found in kidney, spleen, lung, placenta, and ovary, intermediate levels in lymphocytes and muscle, and lower levels in brain, heart, liver, small intestine, and prostate.
▼ Gene Function
Katoh et al. (1998) found that ZK1 mRNA was induced in leukemia cell lines by exposure to ionizing radiation. ZK1 transfection increased apoptotic cell death of murine myeloid precursor 32D cells following irradiation and IL3 (147740) deprivation.
By FISH and radiation hybrid analysis, Katoh et al. (1998) mapped the ZK1 gene to chromosome 19p13.2.