Alternative titles; symbolsSPINDLIN FAMILY, MEMBER 2; SPIN2SPIN2SPIN2, TELOMERIC COPYHGNC Approved Gene Symbol: SPIN2BCytogenetic location: Xp11.21 Genomic c...
Alternative titles; symbols
HGNC Approved Gene Symbol: SPIN2B
Cytogenetic location: Xp11.21 Genomic coordinates (GRCh38): X:57,112,141-57,121,904 (from NCBI)
▼ Cloning and Expression
Using retroviral gene transfer, Fletcher et al. (2002) transformed an IL3 (147740)-dependent murine myeloid cell line with a human myeloid cell line cDNA library. By screening for clones that resisted apoptosis following IL3 removal, they identified SPIN2. The deduced 258-amino acid protein has a C-terminal potential ribonucleotide-binding site and a tyrosine phosphorylation site. SPIN2 and DXF34 (300621) are nearly identical at the nucleotide level and differ primarily in their extreme 5-prime UTRs. The SPIN2 and DXF34 proteins are identical except for 1 amino acid difference. The SPIN2 protein also shows high similarity to human spindlin (SPIN; 609936) and mouse Ssty. RNA dot blot analysis detected SPIN2 in all tissues examined, with highest expression in adult and fetal liver, followed by heart, stomach, kidney, skeletal muscle, placenta, and pancreas. Epitope-tagged SPIN2 localized in the nucleus of a transduced mouse fibroblast cell line. Western blot analysis detected SPIN2 at an apparent molecular mass of 32 kD.
▼ Gene Function
Fletcher et al. (2002) found that human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand (134638) stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.
▼ Gene Structure
Fletcher et al. (2002) identified an intron within the 5-prime UTR of the SPIN2 gene. The promoter region contains numerous Sp1 (189906) sites and a CAATT box.
By genomic sequence analysis, Fletcher et al. (2002) mapped the SPIN2 gene to a region 13 kb telomeric of the DXF34 gene on chromosome Xp11.21.