HGNC Approved Gene Symbol: AK9Cytogenetic location: 6q21 Genomic coordinates (GRCh38): 6:109,492,854-109,691,201 (from NCBI)▼ DescriptionAdenylate kinases, s...
HGNC Approved Gene Symbol: AK9
Cytogenetic location: 6q21 Genomic coordinates (GRCh38): 6:109,492,854-109,691,201 (from NCBI)
Adenylate kinases, such as AK9, are phosphotransferases that provide nucleotide diphosphates and triphosphates in response to energy demands and for DNA and RNA synthesis (summary by Amiri et al., 2013).
▼ Cloning and Expression
By database analysis and PCR, Amiri et al. (2013) cloned human AK9. The deduced 421-amino acid protein has a calculated molecular mass of 48.5 kD and contains a Walker A motif. Database analysis revealed high AK9 expression in pituitary, trachea, thymus, testis, and mammary gland, and moderate expression in brain, pharynx, uterus, spleen, and lymph nodes. Fluorescence-tagged AK9 suggested localization to the cytosol and nucleus of transfected HeLa cells.
▼ Gene Function
Amiri et al. (2013) found that recombinant human AK9 catalyzed phosphorylation of AMP, dAMP, CMP, and dCMP with ATP as phosphate donor, whereas mainly CMP was phosphorylated with GTP as phosphate donor. AK9 also showed pronounced nucleoside diphosphate kinase activity with either ATP or GTP as phosphate donor. Amiri et al. (2013) concluded that AK9 has a relatively broad substrate specificity and functions as both a mono- and diphosphate kinase, and that its catalytic activity may be determined by local substrate concentrations.
▼ Gene Structure
Amiri et al. (2013) determined that the AK9 gene contains 12 exons and spans 59.5 kb.
By genomic sequence analysis, Amiri et al. (2013) mapped the AK9 gene to chromosome 6q21.