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HEPATOMA-DERIVED GROWTH FACTOR; HDGF

HEPATOMA-DERIVED GROWTH FACTOR; HDGF

HGNC Approved Gene Symbol: HDGFCytogenetic location: 1q23.1 Genomic coordinates (GRCh38): 1:156,742,106-156,752,447 (from NCBI)▼ Cloning and ExpressionNakamu...

HGNC Approved Gene Symbol: HDGF

Cytogenetic location: 1q23.1 Genomic coordinates (GRCh38): 1:156,742,106-156,752,447 (from NCBI)

▼ Cloning and Expression
Nakamura et al. (1994) purified a novel hepatoma-derived growth factor from the conditioned medium of human hepatoma-derived cell line HuH-7. Molecular cloning of a cDNA from the cDNA library of the same cell line was done on the basis of the N-terminal amino acid sequence. The cDNA was 2.4 kb long and the deduced amino acid sequence contained 240 amino acids without a signal peptide-like N-terminal hydrophobic sequence. The primary sequence shared homology with the high mobility group-1 protein (163905); they showed 23.4% amino acid identity and 35.6% amino acid similarity.

▼ Gene Function
By immunofluorescence study, Nakamura et al. (1994) showed that HDGF is localized in the cytoplasm of hepatoma cells and northern blots showed that it is expressed ubiquitously in normal tissues and tumor cell lines. Nakamura et al. (1994) suggested that it is a novel heparin-binding protein with mitogenic activity for fibroblasts.

▼ Mapping
By PCR screening of a commercial monochromosomal hybrid panel, Wanschura et al. (1996) mapped HDGF to the X chromosome. By FISH, they refined the localization to Xq25. Subsequently, however, the International Radiation Hybrid Mapping Consortium mapped the HDGF gene to chromosome 1 (SHGC-132090). Amberger (2007) refined the localization to 1q21 based on an alignment of the HDGF sequence (GenBank D16431) with the genomic sequence (build 36.2).

Tags: 1q23.1