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FYVE, RhoGEF, AND PH DOMAIN-CONTAINING PROTEIN 3; FGD3

FYVE, RhoGEF, AND PH DOMAIN-CONTAINING PROTEIN 3; FGD3

Alternative titles; symbolsZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 5; ZFYVE5HGNC Approved Gene Symbol: FGD3Cytogenetic location: 9q22.31 Genomic coordinat...

Alternative titles; symbols

  • ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 5; ZFYVE5

HGNC Approved Gene Symbol: FGD3

Cytogenetic location: 9q22.31 Genomic coordinates (GRCh38): 9:92,947,522-93,036,235 (from NCBI)

▼ Description
FGD3 is predicted to function as a guanine nucleotide exchange factor (GEF) for CDC42 (116952) in control of actin cytoskeletal dynamics and filopodia formation at the cell membrane (Pasteris et al., 2000).

▼ Cloning and Expression
Pasteris et al. (2000) cloned mouse and human FGD3 from spleen cDNA libraries. The deduced 733-amino acid mouse protein contains an N-terminal RhoGEF domain, followed by a pleckstrin homology (PH) domain, a FYVE domain, and a second PH domain near the C terminus. The PH domain is predicted to function in cell signaling, and the FYVE domain is predicted to bind phosphatidylinositol 3-phosphate. Fgd3 shares a high degree of domain similarity with Fgd1 (300546) but lacks the Fgd1 N-terminal proline-rich region. Northern blot analysis detected robust expression of a 5.0-kb Fgd3 transcript in adult mouse brain, spleen, lung, and skeletal muscle, but not in heart, liver, kidney, or testis. A 5.4-kb transcript was also observed in these mouse tissues. The 5.0-kb transcript was observed at all embryonic mouse stages examined, with highest expression at embryonic day 11.

▼ Gene Function
Pasteris et al. (2000) found that mouse Fgd3 produced dramatic and rapid induction of filopodia extensions following microinjection in Swiss 3T3 cells. Fgd3 did not induce formation of stress fibers or overt lamellipodia, similar to Fgd1, which functions as a GEF for Cdc42.

▼ Mapping
By PCR of a human-hamster hybrid panel and radiation hybrid analysis, Pasteris et al. (2000) mapped the FGD3 gene to chromosome 9q22. They mapped the mouse Fgd3 gene to the proximal end of chromosome 13, which shares homology of synteny with human chromosome 9q22.

Hartz (2017) mapped the FGD3 gene to chromosome 9q22.31 based on an alignment of the FGD3 sequence (GenBank AK000004) with the genomic sequence (GRCh38).

Tags: 9q22.31