全周 (9AM - 6PM)

我们和你在一起

Extra info thumb
DIACYLGLYCEROL LIPASE, BETA; DAGLB

DIACYLGLYCEROL LIPASE, BETA; DAGLB

Alternative titles; symbolsDAGL-BETADGL-BETADGLBHGNC Approved Gene Symbol: DAGLBCytogenetic location: 7p22.1 Genomic coordinates (GRCh38): 7:6,409,128-6,447,...

Alternative titles; symbols

  • DAGL-BETA
  • DGL-BETA
  • DGLB

HGNC Approved Gene Symbol: DAGLB

Cytogenetic location: 7p22.1 Genomic coordinates (GRCh38): 7:6,409,128-6,447,953 (from NCBI)

▼ Description
DAGLB is a diacylglycerol (DAG) lipase (EC 3.1.1.34) that catalyzes the hydrolysis of DAG to 2-arachidonoyl-glycerol, the most abundant endocannabinoid in tissues. In brain, DAGL activity is required for axonal growth during development and for retrograde synaptic signaling at mature synapses (summary by Bisogno et al., 2003).

▼ Cloning and Expression
By searching the human genome for sequences similar to Penicilium Dagl, Bisogno et al. (2003) identified DAGLA (614015) and DAGLB. The deduced full-length 672-amino acid DAGLB protein contains 4 transmembrane domains near its N terminus, followed by a serine lipase motif, a lipase-3 motif, and a C-terminal tail. The N terminus and catalytic domain were predicted to reside inside the cell. Mouse and human DAGLB share 79% amino acid identity. Dagla and Daglb were coexpressed in axonal tracts of embryonic mouse at embryonic day 10, but were absent from axonal tracts in adult mouse brain. In adult cerebellum, Dagla and Daglb expression was restricted to deep cerebellar nuclei and synaptic fields, including Purkinje cell dendrites.

▼ Gene Function
Using sn-1-steroyl-2-arachidonoyl-glycerol as substrate, Bisogno et al. (2003) showed that the membrane fraction of COS cells transfected with mouse Daglb showed optimal activity at pH 7. Daglb showed a 3- to 8-fold selectivity for the sn-1 over the sn-2 position of DAGs, and it appeared to prefer sn-1-oleoyl-2 acyl-glycerols with linoleic acid more than oleic, arachidonic, or stearic acids in the 2 position. Daglb was sensitive to ser/cys-hydrolase inhibitors and to a drug that blocked 2-arachidonoyl-glycerol synthesis. Glutathione and calcium stimulated Daglb activity.

▼ Mapping
Hartz (2011) mapped the DAGLB gene to chromosome 7p22.1 based on an alignment of the DAGLB sequence (GenBank AF450090) with the genomic sequence (GRCh37).

▼ Animal Model
Tanimura et al. (2010) found that Dglb -/- mice were viable and appeared normal. Histologic examination revealed no brain abnormalities, and synaptic transmission, kinetics, and currents were no different from wildtype. Endocannabinoid-mediated retrograde synaptic suppression was intact in Dglb -/- brain.

Tags: 7p22.1