Alternative titles; symbolsPROTEIN PHOSPHATASE, MAGNESIUM-DEPENDENT, 1JPROTEIN PHOSPHATASE 2C, ZETA ISOFORM; PP2CZPPP2CZHGNC Approved Gene Symbol: PPM1JCytogenet...
Alternative titles; symbols
HGNC Approved Gene Symbol: PPM1J
Cytogenetic location: 1p13.2 Genomic coordinates (GRCh38): 1:112,709,997-112,715,331 (from NCBI)
The protein phosphatase-2C family is 1 of 4 major serine/threonine protein phosphatase families found in eukaryotes; see 176915.
▼ Cloning and Expression
By EST database searching, followed by 5-prime and 3-prime RACE, Kashiwaba et al. (2003) cloned mouse Ppm1j, which the authors called protein phosphatase-2C-zeta. The deduced protein contains 6 motifs conserved among all mammalian PP2C family members. It has a unique N-terminal region and a proline-rich domain between conserved motifs II and III. Recombinant Ppm1j protein showed Mg(2+)- or Mn(2+)-dependent protein phosphatase activity. Northern blot analysis detected a 1.7-kb Ppm1j transcript exclusively in testicular germ cells.
▼ Gene Function
Using Ppm1j as bait in a yeast 2-hybrid analysis of an adult mouse testis library, Kashiwaba et al. (2003) identified ubiquitin-conjugating enzyme-9 (UBE2I; 601661) as a binding partner with Ppm1j. GST pull-down assays in COS-7 cells confirmed the binding of Ppm1j with Ube2i, and showed that this binding is greatly enhanced by small ubiquitin-related modifier-1 (SUMO1; 601912).
Gross (2014) mapped the PPM1J gene to chromosome 1p13.2 based on an alignment of the PPM1J sequence (GenBank BC073828) with the genomic sequence (GRCh38).