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REGULATOR OF G PROTEIN SIGNALING 16; RGS16

REGULATOR OF G PROTEIN SIGNALING 16; RGS16

HGNC Approved Gene Symbol: RGS16Cytogenetic location: 1q25.3 Genomic coordinates (GRCh38): 1:182,598,622-182,604,388 (from NCBI)▼ Cloning and ExpressionBy se...

HGNC Approved Gene Symbol: RGS16

Cytogenetic location: 1q25.3 Genomic coordinates (GRCh38): 1:182,598,622-182,604,388 (from NCBI)

▼ Cloning and Expression

By searching for retinal-specific RGS family members that might be involved in the phototransduction cascade, Chen et al. (1996) identified cDNAs encoding the mouse and rat homologs of RGS16, called Rgs-r by them. Northern blot analysis showed that rat Rgs16 is expressed predominantly in the retina. Chen et al. (1996) found that mouse Rgs16 enhances the rate of GTP-hydrolysis by transducin (see GNAT2; 139340), suggesting that Rgs16 may play a role in regulating the kinetics of signaling in the phototransduction cascade. The mouse and rat Rgs16 proteins have 94% amino acid sequence identity.

Snow et al. (1998) reported that the mouse and human RGS16 proteins have 86% amino acid sequence identity. Members of the 'regulator of G protein signaling' (RGS) gene family encode proteins that stimulate the GTPase activities of G protein alpha-subunits. See 602189 for background. To identify novel p53 (191170) target genes, Buckbinder et al. (1997) used a differential cDNA screening approach. They isolated a cDNA encoding a predicted 202-amino acid protein with the domain characteristics of RGS family members, and they designated it A28-RGS14. Northern blot analysis showed that RGS16 is widely expressed as an approximately 2.4-kb mRNA and that its expression is induced by mitogenic signals. Overexpression of RGS16 inhibits G protein-coupled mitogenic signal transduction and activation of the mitogen-activated protein kinase (MAPK) signaling cascade.

Using Northern blot analysis, Snow et al. (1998) found that RGS16 is expressed at high levels in retina and at lower levels in all other tissues examined.

▼ Gene Structure

Snow et al. (1998) found that the RGS16 gene contains 5 exons.

▼ Gene Function

Using yeast 2-hybrid and GST pull-down assays, Zheng et al. (2000) demonstrated that the RGS domain of RGS16 interacts with MIR16 (605943).

▼ Mapping

By fluorescence in situ hybridization, Snow et al. (1998) mapped the RGS16 gene to 1q25-q31. The RGS1 (600323) and RGS2 (600861) genes have also been mapped to this region.

Tags: 1q25.3