HGNC Approved Gene Symbol: CD2BP2Cytogenetic location: 16p11.2 Genomic coordinates (GRCh38): 16:30,350,772-30,355,307 (from NCBI)▼ Cloning and ExpressionHuma...
HGNC Approved Gene Symbol: CD2BP2
Cytogenetic location: 16p11.2 Genomic coordinates (GRCh38): 16:30,350,772-30,355,307 (from NCBI)
▼ Cloning and Expression
Human CD2 (186990) facilitates both adhesion and activation of T lymphocytes on binding to its ligand CD58 (LFA3; 153420). This binding augments interleukin-12 (IL12; see 161560)-driven T-cell responsiveness. By use of interaction trap cloning with the yeast 2-hybrid system and 5-prime RACE PCR, Nishizawa et al. (1998) identified a T-cell line cDNA encoding a 341-amino acid protein, termed CD2BP2, that interacts with the cytoplasmic tail of CD2. The authors noted that a segment near the C terminus contains a consensus site for tyrosine phosphorylation. RNA blot analysis indicated that CD2BP2 is expressed in a wide variety of organs as approximately 1.35- and 4-kb transcripts. Immunoprecipitation experiments showed that CD2BP2 expressed in J77 Jurkat and COS-7 cells associates specifically with CD2. Mutation analysis suggested that a 21-amino acid motif is critical for CD2 binding and that 2 PPPGHR sequences within the CD2 cytoplasmic tail mediate the binding to CD2BP2. Additional transfection studies showed that CD2BP2 expression selectively enhances IL2 (147680) production on crosslinking of CD2.