Alternative titles; symbolsCHROMOSOME 6 OPEN READING FRAME 211; C6ORF211HGNC Approved Gene Symbol: ARMT1Cytogenetic location: 6q25.1 Genomic coordinates (GRC...
Alternative titles; symbols
HGNC Approved Gene Symbol: ARMT1
Cytogenetic location: 6q25.1 Genomic coordinates (GRCh38): 6:151,452,465-151,470,100 (from NCBI)
ARMT1 is a protein-glutamate O-methyltransferase (EC 22.214.171.124) that transfers the methyl group from the cofactor S-adenosyl methionine (SAM) onto protein substrates. ARMT1 may play a role in DNA damage response (summary by Perry et al., 2015).
▼ Cloning and Expression
By performing assays for carboxyl methyltransferase activity in fractionated human breast cancer cells, followed by mass spectrometric and database analyses, Perry et al. (2015) identified ARMT1. The deduced 441-amino acid protein shares significant structural similarity with SAM-dependent methyltransferases.
▼ Gene Function
By assaying recombinant protein expressed in bacteria, Perry et al. (2015) found that ARMT1 showed carboxyl methyltransferase activity directed toward PCNA (176740), which functions as a sliding clamp during DNA replication and repair. ARMT1 also showed automethylation activity that appeared to function in a negative-feedback loop. Knockdown of ARMT1 via short hairpin RNA sensitized SK-Br-3 human breast cancer cells to DNA damage, but it induced resistance to DNA damage in MCF7 human breast cancer cells.
Hartz (2015) mapped the ARMT1 gene to chromosome 6q25.1 based on an alignment of the ARMT1 sequence (GenBank AK022972) with the genomic sequence (GRCh38).