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ZINC FINGER PROTEIN 318; ZNF318

ZINC FINGER PROTEIN 318; ZNF318

Alternative titles; symbolsTESTICULAR ZINC FINGER; TZFZFP318HGNC Approved Gene Symbol: ZNF318Cytogenetic location: 6p21.1 Genomic coordinates (GRCh38): 6:43,...

Alternative titles; symbols

  • TESTICULAR ZINC FINGER; TZF
  • ZFP318

HGNC Approved Gene Symbol: ZNF318

Cytogenetic location: 6p21.1 Genomic coordinates (GRCh38): 6:43,336,069-43,369,646 (from NCBI)

▼ Description
ZNF318 is predicted to function in transcriptional regulation (Ishizuka et al., 2005).

▼ Cloning and Expression
Inoue et al. (2000) cloned mouse Znf318, which they called Tzf. The deduced 942-amino acid protein has a calculated molecular mass of 106.5 kD. It has 3 basic amino acid-rich motifs near the N terminus, followed by a myosin II (see MYH9, 160775)-homology domain containing an additional basic amino acid-rich motif, and a C2H2-type zinc finger at the C terminus. The entire protein is rich in proline, serine, and threonine residues, and the C terminus is rich in lysine as well as serine residues. Northern blot analysis detected a 3.8-kb transcript that was predominantly expressed in mouse testis, with little to no expression in 10 other tissues examined. Tzf expression in mouse testis began approximately 10 days after birth and increased corresponding with the appearance of pachytene spermatocytes at meiotic prophase. RT-PCR detected Tzf expression in pachytene spermatocytes, round spermatids, and elongated spermatids.

Using fluorescence-tagged proteins, Ishizuka et al. (2005) localized Tzf to nuclear foci in transfected cells. Tzf-containing foci were distinct from Sc35 (SRSF2; 600813)-containing splicing factor foci.

Tao et al. (2006) stated that alternative splicing creates a long isoform of mouse Tzf (Tzf-L), which contains 2,025 amino acids. The N-terminal 902 amino acids of Tzf-L are identical to those of Tzf. Compared to Tzf, Tzf-L has an additional C2H2-type zinc finger motif and poly-pro and poly-glu domains. Both Tzf and Tzf-L were highly expressed in testis, with moderate expression in kidney and ovary, and both colocalized in nuclear foci.

▼ Gene Function
Androgen receptor (AR; 313700) localizes to the cytoplasm and is recruited to nuclear foci upon binding the AR ligand dihydrotestosterone (DHT). AR has both N- and C-terminal transactivation domains, and both are required for full AR transactivation capacity. Using transfected COS-7 cells, Ishizuka et al. (2005) found that DHT recruited mouse Tzf to nuclear foci in addition to AR. Tzf interacted with the N-terminal transactivation domain of AR, and overexpression of Tzf in COS-7 or LNCaP human prostate cancer cells decreased AR activity in a ligand-dependent manner. Tzf and the transcriptional corepressor N-CoR (NCOR1; 600849) showed additive inhibition of AR activity.

Tao et al. (2006) found that, in contrast to mouse Tzf, Tzf-L enhanced ligand-induced AR transcriptional activation in a dose-dependent fashion in human P3 prostate cancer cells. Tzf and Tzf-L formed homodimers and heterodimers, and coexpression of Tzf and Tzf-L resulted in transactivation of AR.

▼ Mapping
Hartz (2017) mapped the ZNF318 gene to chromosome 6p21.1 based on an alignment of the ZNF318 sequence (GenBank AB015342) with the genomic sequence (GRCh38).

Tags: 6p21.1