MAP8BPY2-INTERACTING PROTEIN 1; BPY2IP1VCY2-INTERACTING PROTEIN 1; VCY2IP1CHROMOSOME 19 OPEN READING FRAME 5; C19ORF5HGNC Approved Gene Symbol: MAP1SCytogenetic ...
BPY2-INTERACTING PROTEIN 1; BPY2IP1
VCY2-INTERACTING PROTEIN 1; VCY2IP1
CHROMOSOME 19 OPEN READING FRAME 5; C19ORF5
HGNC Approved Gene Symbol: MAP1S
Cytogenetic location: 19p13.11 Genomic coordinates (GRCh38): 19:17,719,480-17,734,513 (from NCBI)
▼ Cloning and Expression
Using the SEC1 homology domain of LRPPRC (607544) as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library. The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.
▼ Gene Function
Wang et al. (2012) found that epitope-tagged human nemitin (WDR47; 615734) was expressed diffusely in the cytoplasm in transfected COS-7 cells. Coexpression with mouse Map8 resulted in redistribution of nemitin to the microtubule network. Domain analysis revealed that the C-terminal WD40 domain of nemitin interacted with the N-terminal domain of proteolytically processed Map8 light chain.
Gross (2014) mapped the MAP1S gene to chromosome 19p13.11 based on an alignment of the MAP1S sequence (GenBank BC080547) with the genomic sequence (GRCh37).