MICROTUBULE-ASSOCIATED PROTEIN 1S; MAP1S

MAP8
BPY2-INTERACTING PROTEIN 1; BPY2IP1
VCY2-INTERACTING PROTEIN 1; VCY2IP1
CHROMOSOME 19 OPEN READING FRAME 5; C19ORF5

HGNC Approved Gene Symbol: MAP1S

Cytogenetic location: 19p13.11 Genomic coordinates (GRCh38): 19:17,719,480-17,734,513 (from NCBI)

▼ Cloning and Expression

Using the SEC1 homology domain of LRPPRC (607544) as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library. The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.

▼ Gene Function

Wang et al. (2012) found that epitope-tagged human nemitin (WDR47; 615734) was expressed diffusely in the cytoplasm in transfected COS-7 cells. Coexpression with mouse Map8 resulted in redistribution of nemitin to the microtubule network. Domain analysis revealed that the C-terminal WD40 domain of nemitin interacted with the N-terminal domain of proteolytically processed Map8 light chain.

▼ Mapping

Gross (2014) mapped the MAP1S gene to chromosome 19p13.11 based on an alignment of the MAP1S sequence (GenBank BC080547) with the genomic sequence (GRCh37).