Alternative titles; symbolsLONG NONCODING RNA SPRY4IT1lncRNA SPRY4IT1SPRIGHTLYHGNC Approved Gene Symbol: SPRY4-IT1Cytogenetic location: 5q31.3 Genomic coordi...
Alternative titles; symbols
HGNC Approved Gene Symbol: SPRY4-IT1
Cytogenetic location: 5q31.3 Genomic coordinates (GRCh38): 5:142,317,619-142,318,321 (from NCBI)
SPRY4IT1 is a long noncoding RNA (lncRNA) derived from an intron of the SPRY4 gene (607984) (Khaitan et al., 2011).
▼ Cloning and Expression
Khaitan et al. (2011) reported that SPRY4IT1 is a 687-nucleotide unspliced, polyadenylated lncRNA transcribed from the second intron of the SPRY4 gene. The SPRY4IT1 region is not conserved beyond primate genomes, and EST expression was not detected in mouse. Computational methods predicted several long hairpins in the secondary structure of SPRY4IT1 that are not significantly conserved in orthologous sequences from 31 eutherian mammals. Quantitative RT-PCR in 20 human tissues, not including skin, showed highest expression of SPRY4IT1 in placenta, kidney, and lung, with lower expression in brain, cervix, liver, small intestine, and trachea, and little to no expression in the remaining tissues. RNA-FISH showed that SPRY4IT1 localized predominantly in cytoplasm of transfected human melanoma cells, with some punctate localization in nucleus.
▼ Gene Function
By microarray and quantitative RT-PCR, Khaitan et al. (2011) discovered upregulation of SPRY4IT1 in melanoma cell lines compared with melanocyte control lines. SPRY4IT1 was also upregulated in melanoma patient samples compared with melanocyte controls. Knockdown of SPRY4IT1 via small interfering RNA in human melanoma cells resulted in defects in cell growth, decreased invasion and mobility, and increased apoptosis. Overexpression of SPRY4IT1 in melanoma cells increased the speed of migration by scratch assay.
Gross (2017) mapped the SPRY4IT1 gene to chromosome 5q31.3 based on an alignment of the SPRY4IT1 sequence (GenBank NR_131221) with the genomic sequence (GRCh38).
Khaitan et al. (2011) reported that SPRY4IT1 is located within intron 2 of the SPRY4 gene.